Composite

Part:BBa_K2073006:Design

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


kit1 fac-dex1+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 691
    Illegal AgeI site found at 427
    Illegal AgeI site found at 551
    Illegal AgeI site found at 770
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1788


Design Notes

If bacteria show GFP fluorescence, we could know the fac-dex1 is successfully expressed.


Source

BBa_J04500+BBa_K2073000+BBa_E0040+BBa_B0012

References